RESUMO
OBJECTIVE: To compare the levels of interleukin (IL)-1beta, IL-6, and matrix metalloproteinase (MMP)-8 in the vaginal secretions of pregnant women with a positive fetal fibronectin (fFN) test result with or without asymptomatic bacterial vaginosis (BV) before and after treatment with oral clindamycin. METHODS: A prospective cohort study was conducted among 43 pregnant women with a positive fFN test result. All patients were treated with clindamycin, and the pre- and post-treatment levels of IL-1beta, IL-6, and MMP-8 were compared. RESULTS: Before treatment, levels of IL-1beta and MMP-8 were significantly higher in women with BV compared with women without BV (P<0.05). Vaginal levels of IL-1beta and IL-6, but not MMP-8, decreased after treatment in pregnant women with BV. CONCLUSIONS: The inability of clindamycin to decrease MMP-8 vaginal levels may explain why it is ineffective in reducing preterm birth in pregnant women with positive fFN and BV.
Assuntos
Antibacterianos/farmacologia , Clindamicina/farmacologia , Metaloproteinase 8 da Matriz/efeitos dos fármacos , Vaginose Bacteriana/tratamento farmacológico , Adulto , Antibacterianos/uso terapêutico , Clindamicina/uso terapêutico , Estudos de Coortes , Feminino , Fibronectinas/análise , Humanos , Inflamação/tratamento farmacológico , Inflamação/etiologia , Inflamação/metabolismo , Interleucina-1beta/efeitos dos fármacos , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Metaloproteinase 8 da Matriz/metabolismo , Gravidez , Complicações Infecciosas na Gravidez/tratamento farmacológico , Complicações Infecciosas na Gravidez/fisiopatologia , Nascimento Prematuro/etiologia , Nascimento Prematuro/prevenção & controle , Estudos Prospectivos , Vagina/metabolismo , Vagina/microbiologia , Vaginose Bacteriana/complicações , Vaginose Bacteriana/fisiopatologia , Adulto JovemRESUMO
OBJECTIVE: To compare matrix metalloproteinase (MMP)-8 and MMP-9 levels in the vaginal secretions of pregnant women with or without asymptomatic bacterial vaginosis (BV). METHODS: In this study, vaginal levels and molecular forms of MMP-8 and MMP-9 were studied in 36 pregnant women between 28 and 34 weeks of gestation with asymptomatic BV and 41 pregnant women, matched for gestational age, without BV. RESULTS: Vaginal MMP-8 concentrations were significantly higher (P = .023) in BV-positive women. There was no significant difference in MMP-9 levels between healthy pregnant controls and BV-positive pregnant women. The presence of MMP-8 was confirmed by a 38-kd band on Western blots. CONCLUSIONS: Our findings show that BV is associated with increased levels of MMP-8 in vaginal fluid. Increased production of collagen-degrading enzymes such as MMP-8 is a possible cause of spontaneous preterm delivery in pregnant women with asymptomatic BV.
Assuntos
Metaloproteinases da Matriz/análise , Complicações Infecciosas na Gravidez/enzimologia , Vagina/enzimologia , Vaginose Bacteriana/enzimologia , Adulto , Western Blotting , Ensaio de Imunoadsorção Enzimática , Feminino , Idade Gestacional , Humanos , Metaloproteinase 8 da Matriz/análise , Metaloproteinase 9 da Matriz/análise , Trabalho de Parto Prematuro/enzimologia , Paridade , GravidezRESUMO
Interleukin-8 (IL-8) may play a role in the activation of the vaginal immune system during bacterial vaginosis. However, contradictory results were obtained regarding the involvement of IL-8 in the immunological response during bacteria vaginosis. These apparently contradictory results could be due to different genetic variations of the study groups. Since some gene polymorphisms may affect the level of IL-8 production, the aim of this study was to determine whether the frequency IL-8 promoter alleles and levels of IL-8 in vaginal fluid are associated with bacterial vaginosis during pregnancy. Genotyping for IL-8 polymorphisms in the promoter region of the gene was performed in 34 pregnant women with asymptomatic bacterial vaginosis matched for gestational age with 38 pregnant women without vaginosis. Additionally, vaginal IL-8 levels were assayed by the dual monoclonal antibody sandwich enzyme-linked immunosorbent assay technique. The frequencies of the three polymorphisms were not significantly different between control women and women with bacterial vaginosis. In addition, there was no linkage disequilibrium between the polymorphisms. Furthermore, there was no statistical difference in median vaginal levels of IL-8 between both groups. Neither the frequencies of IL-8 polymorphic alleles nor levels of IL-8 in vaginal fluid were associated with bacterial vaginosis.
Assuntos
Interleucina-8/análise , Interleucina-8/genética , Polimorfismo Genético , Complicações Infecciosas na Gravidez/imunologia , Vagina/imunologia , Vaginose Bacteriana/genética , Vaginose Bacteriana/imunologia , Adulto , Feminino , Frequência do Gene , Humanos , Gravidez , Regiões Promotoras Genéticas/genética , Vagina/química , Vagina/microbiologiaRESUMO
Los tuberculomas del sistema nervioso central son lesiones intracraneanos poco frecuentes, aparecen en cualquier sitio del tejido nervioso, sus características clínicas son: cefalea, parálisis espontánea del VI par, crisis convulsivas o hemorragia subaracnoidea espontanea. Otras enfermedades que pueden simular el mismo cuadro son: toxoplasmosis, cisticercosis, infección por VIH o gliomas. Por ser lesiones muy pequeñas y únicas, su identificación por tomografía axial computada tiene baja sensibilidad, no así la resonancia magnética nuclear, con alta especificidad y sensibilidad en el diagnóstico temprano. El tratamiento a base de esteroides y antifímico mejorar notablemente los efectos de masa intracraneana
